mouse igg anti-βactin antibody  (Thermo Fisher)

Journal: The Journal of toxicological sciences

Article Title: Astragaloside IV protects against cisplatin-induced liver and kidney injury via autophagy-mediated inhibition of NLRP3 in rats.

doi: 10.2131/jts.44.167

Figure Lengend Snippet: Fig. 4. Expression of NLRP3 in response to cisplatin and AS IV. (A) Expression levels of NLRP3, IL-1β, and caspase-1 in rat liv- ers by western blot. (B) Expression of NLRP3, IL-1β, and caspase-1 in rat kidneys by western blot. (C) Images of rat liver and kidney immunohistochemistry sections stained for NLRP3 expression (× 400). The relative expression of protein was calculated as band intensity of target protein normalized to β-actin. *p < 0.05, **p < 0.01, and ***p < 0.001. (n = 4).

Article Snippet: The antibodies used in this study included rabbit anti-NLRP3 (Bioss Antibodies, Beijing, China), rabbit anti-caspase-1 (Abcam, Cambridge, MA, USA), mouse anti-IL-1β (Santa Cruz, CA, USA), rabbit anti-LC3 (CST, Beverly, MA, USA), mouse antiSQSTM1/p62 (Santa Cruz, CA, USA), and rabbit anti-βactin (CST).

Techniques: Expressing, Western Blot, Immunohistochemistry, Staining

Journal: The Journal of toxicological sciences

Article Title: Astragaloside IV protects against cisplatin-induced liver and kidney injury via autophagy-mediated inhibition of NLRP3 in rats.

doi: 10.2131/jts.44.167

Figure Lengend Snippet: Fig. 5. Expression levels of LC 3 II/I and p62 in response to cisplatin and AS IV. (A) Expression levels of LC3 II/I and p62 in rat livers, as measured by western blot. (B) Expression of LC3 II/I and p62 in rat kidneys, as measured by western blot. (C) Images of rat liver and kidney immunohistochemistry sections stained for p62 expression (× 400). The relative conversion of LC3 II/I was calculated as the band intensity of LC3 II normalized to LC3 I. The relative expression of p62 was calcu- lated as the band intensity of p62 normalized to β-actin. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group. (n = 4).

Article Snippet: The antibodies used in this study included rabbit anti-NLRP3 (Bioss Antibodies, Beijing, China), rabbit anti-caspase-1 (Abcam, Cambridge, MA, USA), mouse anti-IL-1β (Santa Cruz, CA, USA), rabbit anti-LC3 (CST, Beverly, MA, USA), mouse antiSQSTM1/p62 (Santa Cruz, CA, USA), and rabbit anti-βactin (CST).

Techniques: Expressing, Western Blot, Immunohistochemistry, Staining, Control